Laboratorio de Fisiopatogenia

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IbarraDirectora

Dra Cristina Ibarra

Prof. Titular, Facultad de Medicina, UBA

Investigadora Principal CONICET

Ibarra@fmed.uba.ar/ 54-11-52853281

 

Ibarra María Marta AmaralDra María Marta Amaral

Jefe de Trabajos Prácticos, Facultad de Medicina, UBA. Investigadora Asistente CONICET

Acción de Stx2 en el riñón. Uso de fármacos para prevenir el daño renal.

mamaral@fmed.uba.ar/ 54-11-52853280

 

 

Dra Flavia Sacerdoti

Ayudante de Primera, Fac. de Farmacia y Bioquímica, UBA. Investigadora Asistente CONICET.  

Desarrollos innovadores para el diagnóstico, tratamiento y prevención del Síndrome Urémico Hemolítico causado por Escherichia coli productor de toxina Shiga. 

flasacerdoti@gmail.com/54-11-52853280

 

Temas de interés

Nuestro grupo de investigación estudia los mecanismos fisiopatológicos que desencadenan las diarreas producidas por Escherichia coli enterohemorrágica (EHEC) y la acción de la toxina Shiga (Stx2) y otros factores de virulencia responsables del Síndrome Urémico Hemolítico (SUH) con el propósito de desarrollar nuevas estrategias para disminuir la incidencia de EHEC y el desarrollo del SUH.

Integrantes

Lic. Adriana Albanese Estudiante de Doctorado Facultad de Medicina- UBA [+]
Lic Romina Álvarez Estudiante de Doctorado Facultad de Medicina- UBA [+]
Lic. Nicolás Garimano Estudiante de Doctorado, FCEN-UBA [+]
Lic. M. Luján Scalise Estudiante de Doctorado, Facultad de Medicina, UBA [+]

Sección Patología

Ibarra Elsa ZottaDra Elsa Zotta

Director del grupo

Profesora Adjunta. Facultad de Medicina, UBA

Investigadora Universidad de Buenos Aires

ezotta@fmed.uba.ar/ 54-11-52853317

 

Integrantes

Dr Federico Ochoa Ayudante de Primera. Facultad de Medicina. UBA [+]
Bioq. y Farm. Abril Seyahian Docente FFyB UBA, Becaria Doctoral CONICET [+]

Nuestro grupo de investigación tiene como finalidad estudiar los mecanismos fisiopatológicos involucrados en la evolución de la insuficiencia renal aguda a crónica en un modelo animal de SUH y evaluar las alteraciones renales durante la progresión a la cronicidad en modelos animales de diabetes.

Publicaciones destacadas

    1. Clade 8 and clade 6 strains of Escherichia coli O157:H7 from cattle in Argentina have hypervirulent-like phenotypes. Amigo N, Mercado E, Singh P, Vilte D, Gerhardt E, Zotta E, Ibarra C, Manning SD, Larzábal M, Cataldi A. Plos One 2015, 10: e127710. Link

     

    1. Impact of infection dose and previous serum antibodies against the locus of enterocyte effacement proteins on Escherichia coli O157:H7 shedding in calves following experimental infection. Martorelli L, Hovde CJ, Vilte DA, Zotta E, Ibarra C, Cantet RJC, Mercado EC, Cataldi A. BioMed Res Internat 2015, 2015: Link

     

    1. Prevention of renal damage caused by Shiga toxin type 2. Action of Miglustat on human endothelial and epithelial cells. Girard MC, Sacerdoti F, Rivera FP, Repetto HA, Ibarra C, Amaral MM. Toxicon 2015, 105: 27-33. Link

     

    1. Copious Podocyturia without Proteinuria and with Normal Renal Function in a Young Adult with Fabry Disease.Trimarchi H, Canzonieri R, Muryan A, Schiel A, Araoz A, Forrester M, Karl A, Lombi F, Andrews J, Pomeranz V, Rengel T, Zotta E. Case Rep Nephrol. 2015; 2015: 257628. Link

     

    1. Comparative characterization of Shiga toxin type 2 and Subtilase cytotoxin effects on human renal epithelial and endothelial cells grown in monolayer and bilayer conditions. Alvarez RS, Sacerdoti F, Jancic C, Paton AW, Paton JC, Ibarra C, Amaral MM. Plos One 2016, 11(6): e0158180. Link

     

    1. Immunization with BLS-Stx2B chimera totally protects dams from early pregnancy loss induced by Shiga toxin type 2 (Stx2) and confers anti-Stx2 immunity to the offspring. Sacerdoti F, Mejías MP, Bruballa AC, Álvarez RS, Amaral MM, Palermo MS, Ibarra C. Vaccine 2016, 34: 4732-4737. Link 

     

    1. Podocyturia: A Clue for the Rational Use of Amiloride in Alport Renal Disease. Trimarchi H, Canzonieri R, Muryan A, Schiel A, Araoz A, Paulero M, Andrews J, Rengel T, Forrester M, Lombi F, Pomeranz V, Iriarte R, Zotta E. Case Rep. Nephrol. 2016; 2016:1492743. Link

     

    1. Podocyturia is significantly elevated in untreated vs treated Fabry adult patients. Trimarchi H, Canzonieri R, Schiel A, Politei J, Stern A, Andrews J, Paulero M, Rengel T, Aráoz A, Forrester M, Lombi F, Pomeranz V, Iriarte R, Young P, Muryan A, Zotta E. J. Nephrol. 2016 Feb 3. Link

     

    1. Increased urinary CD80 excretion and podocyturia in Fabry disease.Trimarchi H, Canzonieri R, Schiel A, Costales-Collaguazo C, Politei J, Stern A, Paulero M, Rengel T, Andrews J, Forrester M, Lombi M, Pomeranz V, Iriarte R, Muryan A, Zotta E, Sanchez-Niño MD, Ortiz A. J Transl Med. 2016 Oct 13;14(1):289. Link

     

    1. Immune Response in Calves Vaccinated with Type Three Secretion System Antigens and Shiga Toxin 2B Subunit of Escherichia coli O157:H7. Martorelli L, Garbaccio S, Vilte DA, Albanese AA, Mejías MP, Palermo MS, Mercado EC, Ibarra C, Cataldi A. Plos One 2017, 12 (1): e0169422. Link

     

    1. Systemic effects of Subtilase cytotoxin produced by Escherichia coli O113:H21. Seyahian EA, Oltra G, Ochoa F, Melendi S, Hermes R, Paton JC, Paton AW, Lago N, Castro Parodi M, Damiano A, Ibarra C, Zotta E. Toxicon 2017, 127: 49. Link

     

    1. Microbiologic control and serodiagnosis of Escherichia coli O157:N7 on kindergartens’staff in Buenos Aires city and suburban areas. Fernandez Brando RJ, Amaral MM, Ciocchini AE, Bentancor LV, Trelles JA, Da Rocha M, Landriel M, Ugarte M, Briones G, Ibarra C, Palermo MS. Medicina (Buenos Aires) 2017, 77: 185-190. Link

     

    1. Shiga Toxin-producing Escherichia coli (STEC) O22:H8 isolated from cattle reduces coli O157:H7 adherence in vitro and in vivo. Martorelli L, Albanese A, Vilte DA, Cantet R, Bentancor A, Zolezzi G, Chinen I, Ibarra C, Rivas M, Mercado EC, Cataldi A. Vet Microbiol 2017, 208: 8-17. Link

     

    1. Ouabain protects human renal cells against the cytotoxic effects of Shiga toxin type 2 and Subtilase cytotoxin.Amaral MM, Girard MC, Álvarez RS, Paton AW, Paton JC, Repetto HA, Sacerdoti F, Ibarra C. Toxins (Basel) 2017 Jul 18; 9(7). Link 

     

    1. Soluble CD40 ligand and oxidative response are reciprocally stimulated during Shiga toxin-associated Hemolytic Uremic Syndrome. Abrey Recalde MJ, Alvarez RS, Alberto F, Mejias MP, Ramos MV, Fernandez Brando RJ, Bruballa AC, Exeni RA, Alconcher L, Ibarra C, Amaral MM, Palermo MS. Toxins (Basel) 2017 Oct 25;9(11). Link

     

    1. Expression of uPAR in Urinary Podocytes of Patients with Fabry Disease. Erratum to: Podocyturia is significantly elevated in untreated vs treated Fabry adult patients. Trimarchi H, Canzonieri R, Schiel A, Politei J, Costales-Collaguazo C, Stern A, Paulero M, Rengel T, Valiño-Rivas L, Forrester M, Lombi F, Pomeranz V, Iriarte R, Muryan A, Ortiz A, Sanchez-Niño MD, Zotta E. Int J Nephrol. 2017, 2017:1287289. Link

     

    1. A dioxin-like compound induces hyperplasia and branching morphogenesis in mouse mammary gland, through alterations in TGF-β1 and aryl hydrocarbon receptor signaling. Miret N, Rico-Leo E, Pontillo C, Zotta E, Fernández-Salguero P, Randi A. Toxicol Appl Pharmacol. 2017, Nov 1;334:192-206. Link

     

    1. Influence of Normo- and Hypogonadal Condition, Hyperuricemia, and High-Fructose Diet on Renal Changes in Male Rats. Soutelo J, Alejandra Samaniego Y, Zotta E, Cecilia Fornari M, Reyes Toso C, Juan Ponzo O. Int J Endocrinol. 2017;2017:1623597. Link

     

    1. In IgA Nephropathy, Glomerulosclerosis Is Associated with Increased Urinary CD80 Excretion and Urokinase-Type Plasminogen Activator Receptor-Positive Podocyturia.Trimarchi H, Canzonieri R, Schiel A, Costales-Collaguazo C, Stern A, Paulero M, Rengel T, Andrews J, Iotti A, Forrester M, Lombi F, Pomeranz V, Iriarte R, Muryan A, Zotta E. Nephron Extra. 2017 May 16;7(2):52-61. Link

     

    1. Low molecular weight hyaluronan induces migration of human choriocarcinoma JEG-3 cells mediated by RHAMM as well as by PI3K and MAPK pathways. Mascaró M, Pibuel MA, Lompardía SL, Díaz M, Zotta E, Bianconi MI, Lago N, Otero S, Jankilevich G, Alvarez E, Hajos SE. Histochem Cell Biol. 2017 Aug;148(2):173-187. Link

     

    1. Immunization of pregnant cows with Shiga toxin-2 induces high levels of specific colostral antibodies and lactoferrin able to neutralize coli O157:H7 pathogenicity. Albanese A, Sacerdoti F, Seyahian E, Amaral MM. Florentino GA, Fernandez Brando R, Vilte DA, Mercado EC, Palermo MS Cataldi A, Zotta E, Ibarra C. Vaccine 2018, 36: 1728-1735 Link

     

    1. Efficacy of a recombinant Intimin, EspB and Shiga toxin 2B vaccine in calves experimentally challenged with Escherichia coli O157:H7. Martorelli L, Garimano N, Fiorentino GA, Vilte DA, Garbaccio SG, Barth SA, Menge C, Ibarra C, Palermo MS, Cataldi A. Vaccine 2018, 36: 3949-3959, 2018. Link

     

    1. Shiga Toxin-producing Escherichia coli Infection during pregnancy. Sacerdoti F, Scalise ML, Burdet J, Amaral MM, Franchi AM, Ibarra C. Microorganisms 2018, Oct 23;6(4). pii: E111. Link

     

    1. Human recombinant Fab fragment neutralizes Shiga toxin type 2 cytotoxic effects in vitro and in vivo. Luz D†, Amaral MM†, Sacerdoti F, Bernal AM, Palermo M, Ibarra C, Fontes Piazza RM. Toxins (Basel) 2018, Dec 2;10(12). Link

     

    1. Relevance of Bacteriophage 933W in the Development of Hemolytic Uremic Syndrome (HUS). Del Cogliano ME, Pinto A, Goldstein J, Zotta E, Ochoa F, Fernández-Brando RJ, Muniesa M, Ghiringhelli PD, Palermo MS, Bentancor LV. Front Microbiol. 2018 Dec 13;9:3104.Link

     

    1. A novel penicillin derivative induces antitumor effect in melanoma cells. Blank V, Bellizzi Y, Zotta E, Cornier PG, Delpiccolo CML, Boggián DB, Mata EG, Roguin LP. Anticancer Drugs. 2018 Jun; 29(5):416-428. Link

     

    1. Early decrease in the podocalyxin to synaptopodin ratio in urinary Fabry podocytes. Trimarchi H, Canzonieri R, Costales-Collaguazo C, Politei J, Stern A, Paulero M, González-Hoyos I, Schiel A, Rengel T, Forrester M, Lombi F, Pomeranz V, Iriarte R, Muryan A, Zotta E. Clin Kidney J. 2019 Feb;12(1): 53-60. Link

     

    1. Trophoblast VIP deficiency entails immune homeostasis loss and adverse pregnancy outcome in mice. Hauk V, Vota D, Gallino L, Calo G, Paparini D, Merech F, Ochoa F, Zotta E, Ramhorst R, Waschek J, Leirós CP. FASEB J. 2019 Feb; 33(2):1801-1810. Link

     

    1. Exposure to environmental concentrations of hexachlorobenzene induces alterations associated with endometriosis progression in a rat model. Chiappini F, Sánchez M, Miret N, Cocca C, Zotta E, Ceballos L, Pontillo C, Bilotas M, Randi A. Food Chem Toxicol. 2019 Jan;123:151-161. Link

     

    1. Podocyturia in paediatric patients with Fabry disease. Liern M, Collazo A, Valencia M, Fainboin A, Isse L, Costales-Collaguazo C, Ochoa F, Vallejo G, Zotta E. Nefrologia 2019 Mar-Apr;39(2):177-183. Link

     

    1. Cytotoxic effects of Shiga toxin-2 on human extravillous trophoblast cell lines. Scalise ML, Amaral MM, Reppetti J, Damiano AE, Ibarra C, Sacerdoti F. 2019 Jan 1. pii: REP-18-0581.R1. Link

Publicaciones completas C Ibarra [+]

Publicaciones completas E Zotta [+]

Divulgación

Actividad de divulgación de Ciencia y Tecnología. Confección de un video para Intramed: “Una mirada al Síndrome Urémico Hemolítico”

Nota en CONICET

Financiamiento

ANPCyT-PICT 2012-0777, desde 2013 hasta 2016.
ANPCyT-PICT 2014-0936, desde 2015 hasta 2018.
UBACyT-ME770, desde 2014 hasta 2017
CONICET-PIP112, desde 2016-2018.
ANPCyT -FONARSEC-FITS SALUD, desde 2012 hasta 2016.
UBACyT 20020130200223BA. 2014-2016
Subsidio a la Investigación en Bioquímica Molecular y Proteómica. CONICET- COLEGIO ORT 2016

 

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